Titanite U-Pb dating is a significant way to investigate the geological processes such as metamorphism and partial melting. Here, we evaluate in situ titanite U-Pb dating method based on three well-known titanite standards (BLR-1, Ontario, Pakistan), using ThermoFisher Scientific iCapRQ quadrupole-inductively coupled plasma-mass spectrometer (Q-ICP-MS) coupled with 193 nm ArF excimer GeoLas HD laser-ablation system. After evaluating the influence of beam diameters, laser energy, and repetition rates on the U-Pb isotopic fractionation, the optimal instrumental conditions were confirmed at 5～8 J/cm²,5～8 Hz,44 μm. Besides, the titanite standards were accurately determined with ages of 1 047±6 Ma for BLR-1, 1 055±6 Ma for Ontario, 21.0±0.6 Ma for Pakistan. Further, we chose the granulitized eclogite and its partial melts from the central Himalaya for titanite U-Pb dating. Two types of titanite were recognized from the Himalayan granulitized eclogite and its partial melts, metamorphic type in eclogite and peritectic type in the leucosome. The metamorphic titanites in granulitized eclogites are characterized with lower Nb, Ta, Y but higher Nb/Ta. The U-Pb ages for three sets of metamorphic titanites are 14.1±1.6, 14.2±0.4 and 15.3±2.5 Ma. However, titanite commonly appears as the retrograded product and records the cooling age in eclogite, hence this study insists that granulitized eclogite from the central Himalaya has retrograded into granulite facies to high amphibolite facies at 15～14 Ma, combined with the Zr thermometer (700～850℃) of the titanite. The peritectic titanites with melt inclusions occurred are characterized with higher Th, Nb, Ta, REE and Th/U ratio but lower Nb/Ta ratio than the metamorphic type. Forty laser analyses for the peritectic titanite yield intercept ages of 12.1±1.7 Ma, consistent with the zircon rims (13.5±0.9 Ma) from the leucosome, but much younger than the zircon mantle (17.3±3.2 Ma), these results indicate eclogite from the central Himalaya has undergone decompression melting during the middle Miocene. Two different types of titanite U-Pb dating limit the retrograde metamorphism time of granulite facies to high amphibolite facies at 15～14 Ma and the partial melting age at about 12 Ma during the exhumation process.