The Jurassic-Cretaceous Tunxi basin in southern Anhui is located at the junction between the NE-trending Middle-Lower Yangtze volcanic belt and the NNE-trending Southeast China volcanic belt. Geochronological and petrogenetic study of the volcanic rocks can provide information regarding the Late Mesozoic tectonic evolution of South China. LA-ICP-MS zircon U-Pb ages and geochemical data of four volcanic rock samples indicate that two volcanic stages are developed in Tunxi basin. The earlier volcanic rocks were formed at 156~152 Ma. These rocks belong to the calc-alkaline series with middle-to-high K content, and are characterized by the REE right-inclined patterns with weak negative Eu anomalies, LILE and LREE enrichment, and HFSE (such as Nb, Ti, P) depletion. In contrast, the late volcanic rocks were formed at 136~130 Ma and are characterized by bimodal volcanic rocks. Acidic components of these rocks belong to the calc-alkaline series with intermediate K content. These rocks exhibit the right-inclined "V" type REE patterns with strong negative Eu anomalies, LILE and LREE enrichment, and HFSE (such as Nb, Ti, P) depletion. Late Jurassic-Early Cretaceous volcanism features of Tunxi basin resemble those of Southeast China volcanic belt. Combined with previous studies, Late Jurassic volcanic rocks in Tunxi basin were generated in a compressional tectonic environment, whereas Early Cretaceous ones were generated in an extensional tectonic environment. Both of them were attributed to the subductional angular variation and the rollback of the Paleo-Pacific plate from Late Jurassic to Early Cretaceous.