Beryl is an important Be-Al silicate mineral with high economic and industrial value. Laojunshan gneiss dome is located in the joint of South China block and Indosinian block, which has experienced multi-stage tectonic-magmatic action and developed Sn, Cu, Zn and other polymetallic deposits and emerald deposits. This study focuses on the detailed analysis of macro- and micro-structural characteristics of beryl and its symbiotic quartz veins in the region, and limits the temperature environment of formation and deformation through fluid inclusions and quartz EBSD fabric. The results show that beryl and quartz are rich in fluid inclusions, in which beryl has low temperature-high salinity two-phase and high temperature-low salinity three-phase fluid inclusions, and quartz has low temperature-low salinity two-phase fluid inclusions. The deformation characteristics of the quartz associated with beryl mainly develop rhombal <a> superimposed by secondary prim <c>, indicating low-temperture (350～400℃) deformation environment, superimposed by local high-temperture deformation. The magmatic fluid in the middle and late Yanshanian was accompanied by the uplift/exhumation of gneiss dome, which brought rich ore-forming elements, including the main Be elements of beryl mineralization. The exhumation process of regional gneiss dome from deep to shallow provides an important structural ore-bearing fluid channel for mineralization. With the occurrence of fluid immiscibility, the pH value of ore-forming fluid increases, thus promoting the occurrence and precipitation of ore-forming elements.