In order to explore the influence of natural clay minerals and organic matter on the migration and retention of nano emulsified oil in porous media, kaolinite and montmorillonite, as well as humic acid, a typical representative of organic matter, were selected to carry out a batch of experiment on the adsorption of nano emulsified oil by single mineral, organic matter and organic mineral complex. The adsorption mechanism was discussed by means of full analysis of specific surface area, scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR),X-ray diffraction (XRD) and other techniques. The experimental results showed that the adsorption of nano emulsified oil by the media was in accordance with Freundlich model. The adsorption capacity of single mineral and humic acid to nano emulsified oil is as follows:montmorillonite>humic acid>kaolinite, the adsorption capacity of organic mineral composite samples is as follows:montmorillonite-humic acid>kaolinite-humic acid, which are larger than their corresponding single samples, and the phenomenon of "1+1>2" appears, indicating that the more complex the medium composition is, the greater the degree of adsorption and retention of nano emulsified oil is. Further analysis confirmed that nano emulsified oil was mainly adsorbed on the surface of minerals and humic acid through hydrogen bond and hydrophobicity, and the surface structure and properties are the dominant factors in the adsorption process of kaolinite and montmorillonite, so montmorillonite has stronger adsorption capacity, and the adsorption of humic acid is mainly realized through the aggregation between particles. For the composite samples, the adsorption is mainly realized by hydrogen bonding, ligand exchange and hydrophobicity. Combination of humic acid and mineral can increase the sorption sites and enhance the hydrophobicity of mineral surface, thus promoting the sorption. The co-adsorption and cumulative adsorption of humic acid and nano emulsified oil increased with the increase of emulsified oil concentration, which made the adsorption promotion of kaolinite humic acid stronger. With the increase of the concentration of emulsified oil, the humic acid and sorption sites that play the role of "bridge" decreased, which weakened the promoting effect of montmorillonite. The experimental results lay a foundation for further evaluation of migration and retention of nano emulsified oil in porous media.