In this research, mineral inclusions in emeralds from Malipo, Yunnan Province, are studied by polarized microscope, electron probe(EPMA), and cathodoluminescence(CL). The results show that there are several kinds of mineral inclusions which can be considered as origin characteristics in emeralds from Malipo, Yunnan Province, such as black dravite, vanadium muscovite, zonal feldspar, arsenopyrite, magnesium biotite, scheelite. Both in pegmatite veins and shear zone veins, emeralds contain feldspar, albite, dravite, fluorite, emerald (beryl), biotite, sphene, apatite, pyrite, chlorite, epidote. Inclusions only appear in emeralds from pegmatite veins are muscovite, calcite, quartz, zircon, arsenopyrite, sphalerite, galena, diopside. Inclusion only appears in emeralds from shear zone veins is scheelite. This research on different inclusions in emeralds from pegmatite veins and shear zone veins is meaningful not only for emerald identification of origin, but also reveals the genetic type of Yunnan emeralds.