The values of Fe, Cd, Mn and other trace elements in sphalerite not only influence its cell parameters but also contain rich information concerning the genesis of the ore. There are two types of ore in the Dingjiashan Pb-Zn ore district of the Meixian orefield in central Fujian Province: the magnetite-type and the pyrrhotite-type. In this paper, the cell parameters and values of Zn, Fe, Cd and Mn of sphalerite in two types of ore were tested by X-ray in situ and electron microprobe analysis. In terms of the cell parameters of sphalerite, the characteristics of pyrrhotite-type > magnetite-type > the theoretical value are mainly caused by different values of Fe and Mn in sphalerite. In addition, the gap of Fe average values in sphalerite in two types of ore is much larger, and the substitutional relation and intensity between Zn, Fe and Cd of sphalerite in two types of ore are completely different. Combined with the geological characteristics of the study area, the authors consider that these two types of ore may be products of two kinds of mineralization.