Bitumen, natural copper and quartz generally occur in the interstitial site of mineral grains in dark mass in amygdaloidal basalt from Puge County, southern Sichuan. Natural copper formed after the decomposition of organic matter into bitumen and gases. The authors detected that the content of bitumen and that of natural copper exhibit negative correlation. In the dark clumps, the higher the bitumen and quartz, the lower the copper, but the higher the natural copper and quartz in nearby places, and the interval of crystallization time and space between natural copper and bitumen is very short. The commonly recognized sequence is quartz →bitumen→ native copper. The carbon isotope and infrared spectrum suggest that bitumen is of biogenesis and originated from the carbonate rocks in Permian strata. The results from the homogenization temperature of inclusions in quartz and Raman spectrum indicate that the organic matter in the hydrothermal fluids can be decomposed into bitumen and natural gas (CH₄) under the temperature >350℃; the higher the temperature, the higher the maturity of organic matter, the higher the content of organic carbon in bitumen, the lower the natural copper content in residual bitumen.