In this paper, the authors conducted a systematic study of the features of chemical components of different stages of sphalerite in the Huize lead-zinc deposit by using electron microprobe analysis (EMPA) and ICP-MS, so as to explore enrichment regularity and indicative significance of Fe, Zn, Cd, Ge, In and Ag. The results show that hydrothermal metallogenesis of the Huize deposit can be divided into four ore-forming stages, forming fine-grained pyrite, polymetallic sulfides, pyrite-galena and carbonate respectively. Sphalerite can be divided into two generations varying in color from black to red gradually. The early stage sphalerite exhibits enrichment of Fe and depletion of Zn, Cd, Ge, In and Ag. In contrast, the late stage sphalerite shows relative depletion of Fe and enrichment of Zn, Cd, Ge, In and Ag. The content of Cd and Ge has met the requirement of comprehensive utilization, and these two elements are mainly in the form of isomorphism in the sphalerite. Ore-forming temperatures range from 170 to 262℃ at the early stage and below 203 ℃ at the late stage, as shown by the content of FeS in the sphalerite. In combination with the sphalerite Zn/Cd ratios, it is suggested that the Huize lead-zinc deposit is a mesothermal deposit. Spatially, the flowing direction of the hydrothermal fluid was consistent with the strike of the orebodies. Compared with typical lead-zinc deposits, the In, Cd, Ga, Ge, Zn/ Cd, Ga/In and Ge/In of sphalerite in the Huize lead-zinc deposit show characteristics of Mississippi valley-type deposit.