In this paper, the Jinchuan copper-nickel sulfide flotation concentrates were studied to compare the Co, Ni and Cu extraction results of sulfuric acid leaching process and A.f. leaching process. Further enhancement of metals leaching rates was achieved by bio-chemical leaching and chemical-bioleaching. The results showed that the bio-chemical leaching rates of Co²⁺, Ni²⁺ and Cu²⁺ reached 85.05%,98.32% and 95.31%, respectively. The combination of first bioleaching process and the following chemical acid leaching process facilitated the dissolution of the sulfide and strengthened the leaching effects of Co²⁺, Ni²⁺ and Cu²⁺ from complex sulfide concentrate.