Mossbauer spectrum of natural bafertisite from Jiangsu, China, has been obtained for the first time. The valence states, site populations, coordinations, isomorphous substitution and chemical bond of the iron in the mineral have been studied. The data were fitted successfully with a Least-square fitting com- puter program. The MlSssbauer spectrum consists of two non-symmetrical doublets. Their Mossbauer parameters are the following (at 298 K), for the outer doublet, I. S.=1。12mm/s, Q. S.=2.34mm/s, for the inner doublet, I. S.=1.13mm/s, Q. S.=1.89mm/s. I. S. is relative to a-Fe. Some informations about bafertisite have been obtained with the aid of MBssbauer spectroscopy, (1) No Fe3+ component spectrum was separated. The iron ions in the mineral are basically divalent, and it is consistent with the results of the chemical analysis.(2)Comparing the I. S. values of the mineral and other relative minerals, we obtained that Fe2+ of the mineral is in high spin state, its coordination number is six, and the chemical bond between iron and oxygen is basically ionic bond.(3)The two structure sites(M, andM2) of Fe2+ in bafertisite were justified. It is consistent with the crystal structure which was determined by peng Zhizong et al. The principal factor which in- fluences the Q. S. values is the arrangement of the coordination anions. For M1 site, there are two OH- (or F-)which occupy the opposite positions of the coordination octahedron. However, for M2 site, there is only one OH' (orF-) at the apexes of the octahedron, and its distribution is not symmetrical. The Q. S. value for M2 should be larger than that for M1. So the outer doublet was assigned to the Fe2+ in M2 site, and the inner doublet to the Fe2+ in M1 site.(4)The site populations of Fe2+ in M, and M2 were given by combined chemical analysis and Mossbauer spectroscopy. The results indicate that the distribution of Fee' in bafertisite is ordered, Fe2+ occupies mainly M1 site, and Mn2+ is preferable to occupy M2 site than Fe2+·There is isomorphous substitution between Mn2+ and Fe2+ at Mz site.