The discharge of slag and acid mine wastewater has polluted the farmland around the mining area. In order to simulate the relationship between nitrogen and arsenic in the farmland of the mining area under anaerobic conditions, shake flask experiments were carried out to explore the effect of nitrogen on the form and concentration of arsenic released from arsenical ferrihydrite under anaerobic conditions, column experiments were carried out to explore the effect of rice growth on the form and concentration of nitrogen and arsenic in the farmland. The results showed that under anaerobic conditions, the addition of nitrogen may promote the reduction of As(V) in arsenic contaminated paddy soil, making the concentration of As (Ⅲ) reach up to 396 μg/L. During the nitrogen and arsenic changes in different layers of anaerobic farmland simulated by column experiment, rice can promote the reduction of nitrogen and arsenic and the migration of arsenic down the layers during its growth, making the concentration of As (Ⅲ) reach up to 517 μg/L in the installation. By analyzing the similar anaerobic conditions of the shake flask experiment and the column experiment, As (Ⅲ) and NO^-₂, as As(Ⅴ) and NO^-₃ concentrations in the arsenic concentration, it can be inferred that there is maybe an oxidation-reduction relationship between arsenic and nitrogen, and nitrogen is likely to participate in the oxidation-reduction of arsenic.This study provides a certain reference value for the treatment of arsenic pollution in farmland, and enriches the understanding of prevention and control of arsenic pollution in farmland soil.