The Guaiyu turquoise mining site in Lushi, Henan Province is located in the western margin of the Yiluo Basin. The discovery of the mining site provides important archaeological information for the study of the provenience of turquoise in the early Central Plains. In order to determine the direction of the turquoise from the ancient mine, the Sr and Pb isotopic compositions of Guaiyu turquoise were detected by thermoelectric ionization mass spectrometer (TIMS). Combined with the isotopic data of five major turquoise deposits in Qinling Mountains, the provenience characteristics of turquoise from Guaiyu ancient mining site were explored. The results show that the ⁸⁷Sr/⁸⁶Sr ratio of Guaiyu turquoise is relatively high, which is greater than 0.719 0 on the whole, and higher than that of other turquoise deposit, which can be regarded as one of the characteristics of the Guaiyu turquoise. There is a high degree of overlap among Pb isotopic compositions, however, the ²⁰⁸Pb/²⁰⁴Pb-²⁰⁷Pb/²⁰⁸Pb diagram shows some independent data gathering areas,which can be used to judge the origin of the Guaiyu turquoise. In addition, combination of ²⁰⁸Pb/²⁰⁴Pb-⁸⁷Sr/⁸⁶Sr and ²⁰⁷Pb/²⁰⁸Pb-⁸⁷Sr/⁸⁶Sr also obtained a good discrimination result, and the boundary with other regions is clear without obvious overlap. The Sr and Pb isotope composition is an important evidence to judge the origin of the Guaiyu turquoise.