Stromatolites of Tungussia cf. nodosa Semikhatov and Baikalia lacera semikhatov were discovered for the first time in the Langyashan Formation of the original Jixian System near Shuiquanzigou, Qimantag area, Ruoqiang County, Xinjiang. The stromatolites are mostly nodular and columnar, and their overall morphological characteristics can be compared with the characteristics of Tungussia cf. and Baicalia stromatolites distributed in Neoproterozoic strata in Huaishan, Anhui and Eastern Liaoning, and some stromatolites in Jiawengmen area of East Kunlun, which is equivalent to stromatolite assemblage IV in North China. Through our work, Sm-Nd whole-rock isotopic isochron age of 815±26 Ma from the host rock of stromatolites is obtained. According to the Sm-Nd isochron age, the main characteristics of stromatolites in study area and other areas, the geological time of Langyashan Formation in Qimantag area should belong to the Neoproterozoic Qingbaikouan Period. Their host rock should expose in passive margin of a Precambrian micro-continent block disintegrated from Tarim Craton(?), which was probably assigned to Rodinian Super-Continent. The discovery of the stromatolites and dating results of the host rock provide new evidence for the tectonic evolution and diagenetic environment in western segment of the East Kunlun Mountains.