Anatexis is the main mechanism and key geological process of continental crustal differentiation, element migration and enrichment and migmatization. The Archean basement exposed in the southern Jilin Province, in which felsic leucosome and leucogranites are extensively distributed, widely experienced amphibolite-granulite facies metamorphism and anatexis. The field geological characteristics, correlation and petrographic characteristics of the Archean metamorphic quartz diorite and associated felsic leucosome and orthopyroxene (Opx)- and amphibole (Amp)-bearing pegmatite granite occurred in Helong granite-greenstone terrane in the southern Jilin Province indicate that the metamorphic quartz diorite underwent partial melting and in-situ segregation and accumulation of melt. The integrated analyses of zircon cathodoluminescence (CL) images, in-situ U-Pb geochronology, trace elements and Hf isotope on the metamorphic quartz diorite and pegmatite granite show that they have the same types of magmatic and metamorphic recrystallized zircons, the latter is dominated by metamorphic recrystallized zircons, that the metamorphic quartz diorite has magmatic crystallization age of 2 535 ±14 Ma, and that their magmatic zircons have consistent Hf isotope composition. Their metamorphic recrystallized zircons yield consistent metamorphic ages of 2 495±22 Ma and 2 491±10 Ma, respectively, and have identical Hf isotope compositions, which are consistent with that of magmatic zircons, Th and U contents and Th/U ratios, indicating that both metamorphic recrystallized and magmatic zircons have initial crystalline zircons formed in the same magmatic source, and further demonstrating that the metamorphic quartz diorite experienced metamorphic anatexis at ca. 2.5 Ga and that the Opx- and Amp-bearing pegmatite granite derived from in-situ partial melting of the metamorphic quartz diorite, in combination of the coeval strong and extensive magmatism and amphibolite-granulite facies metamorphism with counterclockwise p-T path at the end of Neoarchean in the North China Craton as well as the southern Jilin Province, which may reflect the thermal effect of mantle material upwelling on the continental crust during formation and stabilization of the craton lithosphere.