The Daping Au deposit is one of the most important large-sized gold deposits in the south of the Ailao Mountain-Honghe River ductile shear zone. Due to the disputes on its mineralization timing and tectonic setting, it's difficult to establish the metallogenic model as well as further explore and exploit gold deposits in this belt. In this paper, LA-ICP-MS was used to study the U-Pb geochronology of hydrothermal zircon from Au-bearing quartz veins and magmatic zircon from diorite and granodiorite vein in the Daping Au deposit. The result shows that the age of hydrothermal zircon is 760±10 Ma, suggesting that there was a Neoproterozoic mineralization event for the Daping gold deposit. The magmatic zircons from the diorite and granodiorite vein yielded ages of 773±9 Ma and 766±9 Ma, respectively. These results coincide with the breakup of the zircons from the diorite and granodiorite veins. Therefore, combined with their consistent ages within the error range, the authors hold that the Neoproterozoic gold mineralization event might have been Rodinia supercontinent and the Jinning-Chengjiang large-scaled volcanic-magmatic event in the Ailao Mountain area. The hydrothermal zircons have similar Hf isotopic composition to those of related intrusions of diorite and granodiorite. Although the Eocene-Oligocene was thought to be a main mineralization period for the Daping gold deposit in previous literature, the authors consider that the Daping gold deposit probably resulted from superimposed mineralization from the Neoproterozoic and Cenozoic magmatic-tectonic-hydrothermal events, as shown by the determination of the Neoproterozoic mineralization event in this study.