Collecting four different types of arsenic-containing minerals in Shimen realgar mining area, using Thiobacillus ferrooxidans(T. f), sulfate reducing bacteria(SR) and Acidiphilium cryptum JF-5(JF-5) to investigate the release of arsenic from primary and secondary arsenic-containing minerals under biological reduction and oxidation conditions to determine the potential risk of arsenic release. Quantitative analysis by ICP-OES showed that the concentration of arsenic in both realgar tailing and orpiment tailing was increasing under the action of the above T. f, SR and JF-5. Arsenic released at 168 h under the action of T. f:orpiment tailing > leachate secondary arsenic-containing tailing > realgar tailing > arsenic containing clip rock tailing. The results of LC-AFS atomic fluorescence spectrometry release showed that:① Under the action of T. f, realgar tailing and orpiment tailing showed very obvious differences; after 1.5 h, the order of releasing As(V) for four arsenic-containing minerals is:arsenic containing clip rock tailing > realgar tailing > leachate secondary arsenic-containing tailing > orpiment tailing; ② Under the action of JF-5 and SR, SR can promote the release of As(Ⅲ) from the orpiment tailing, which is twice as much as JF-5; the orpiment tailing releases As(Ⅲ) reached to 20.64 mg/L (SR) and 9.54 mg/L (JF-5) at 168 h. The order of release of As(Ⅲ) from four arsenic-containing minerals under the action of SR at 96 h was:orpiment tailing > leachate secondary arsenic-containing tailing > arsenic containing clip rock tailing > realgar tailing; the order of release of As(III) by four arsenic-containing minerals after 96 h of action of JF-5 is realgar tailing > orpiment tailing > arsenic containing clip rock tailing > leachate secondary arsenic-containing tailing.