Amber is a kind of precious organic gem that has a long history of utilization as beads and jewelries. There are a lot of amber beads of West Han Dynasty to South & North Dynasty from archaeological sites in the collections of Yangzhou Museum, Hepu Museum and museums in Yunnan Province, Hunan Province and Hubei Province. Most of these amber beads have not been analyzed in lab. This study is focused on the identification of age, material and origin of amber beads through different analytical methods in lab. Methods of typology, outer characteristics analysis through magnifying glass and infrared spectrometer that is non-destructive were employed in the study of 4 pieces of ancient amber beads. The preliminary results show that 4 pieces of ancient samples from Beijing Bead Museum were probably the products of West Han to North & South Dynasty of China, and the material of these samples is amber and has the features of amber from Baltic Sea. This study also suggests that amber was probably an important commercial item of the marine silk road 2000 years ago.