Located in the inner zone of the middle part of the eastern Kunlun orogenic belt, the Shitoukengde mafic-ultramafic intrusion is composed of gabbro-lithofacie, pyroxenite-lithofacie and peridotite-lithofacie. Geological data of field indicate that the emplacement order of each lithofacie is gabbro-lithofacie→pyroxenite-lithofacie→peridotite-lithofacie. This study shows the average content of Ni in spinel is 258×10^-6; the olivine crystals are chrysolite with the forsterite content (Fo) ranging from 81 to 86, the content of Ni in olivine is from 471×10^-6 to 2 279×10^-6 and the average is 1 153×10^-6; En of Orthopyroxene is from 80.35 to 83.89, and the average is 82.51. Based on distribution coefficient of Mg-Fe between olivine and melts, the most Fo-rich olivines indicate that the primary magma of Shitoukengde intrusion belongs to high MgO basaltic magma containing about 10.5％ MgO. This intrusion is derived from 11.2％～14.0％ partial melting of mantle. The study of partial melting, primary magma, structural sign, rock assemblage, direct mineralized markers and mineral assemblage, Ni-content and Fo of olivine shows that the Shitoukengde intrusion has favorable conditions for the formation of copper-nickel deposit and the eastern Kunlun orogenic belt can be important nickel prospective region.