Synchrontron radiation performs much better than conventional light source in that it is capable of examining the mineral phase, crystal structure, morphology and chemical composition of biomineralizaiton samples in a tiny amount. Based on synchrontron radiation, the authors employed several test methods to study human breast cancer calcification, including X-ray diffraction, Fourier transform infrared spectroscopy, X-ray fluorescence and three-dimensional imaging. According to the results, breast cancer calcification is mainly composed of(carbonated) hydroxylapatite, with trace elements like Sr and Zn. The psammoma body holds special layered structure. These results provide mineralogical information for the pathological study and diagnosis of breast cancer.