The Jinchuan copper-nickel sulfide tailings are of large quantities and contain many Ni, Cu and Co valuable metals, suggesting great economic potentialities. A.f. was studied to reveal the effect of the microbial extraction of valuable metals. Samples were collected to interact with A.f. in different periods and then sulfuric acid chemical effects were conducted. The concentrations of Cu, Ni and Co were tested by Inductively Coupled Plasma Atomic Emission Spectrometer (ICP-AES) to characterize the capability of microbial extraction of valuable metals. The results showed that Cu²⁺, Ni²⁺ leaching rates of the tailings after direct chemical sulfuric acid effect were 73.78% and 69.87%, and Co²⁺ leaching rate was 39.57%. In 10 days' interaction between the tailing samples and A.f., the total leaching rates of Cu²⁺, Ni²⁺ could reach over 80% and Co²⁺ total leaching rate could be up to 70%, which indicates that the involvement of A. f. can greatly facilitate Cu²⁺, Ni²⁺, Co²⁺ leaching of Jinchuan tailings.