The low-temperature eclogites from the subduction-accretionary complex in the North Qilian Mountain occur as lenses within blueschists and phengite_schists. Based on REE and trace element analyses as well as Sr-Nd isotopic data, the authors divided the eclogites into two types. Type Ⅰ eclogites are characterized by nearly flat REE normalized patterns and a distinctly positive Eu anomaly, suggesting cumulate protoliths, while Type Ⅱ eclogites have slightly Eu anomalies and an enriched LREE pattern. The values of εNd(t） from Type I eclogite group vary between 2.5 and 6.9, indicating depleted mantle sources. The values of εNd（t） from Type Ⅱ eclogites vary between －3.3 and 1.4.These datasuggest that the protoliths of Type Ⅰ eclogites originated in an oceanic environment. Type Ⅱeclogites show apossible mixing with a continental crust contaminant during the emplacement of the rotoliths, suggesting the probable derivationfrom a continental/oceanic transitional zone or a continental margin.