The rutile U-Pb dating methodology established at the Isotope Laboratory of Tianjin Institute of Geology and Mineral Resources is reported in this paper. Some key problems as various factors affecting U and Pb blanks, the isotopic fractionation effect and the interference isotopic peaks are discussed in detail in this paper, with some measures for improvement of the experimental methodology put forward. Rutiles separated from the ultra-high-pressure metamorphic eclogite in the Dabie Mountain was dated using the improved method, and a precise, accurate and geologically significant age of 218.2±1.2 Ma was obtained.