Fibrous sepiolite has cytotoxicity to human erythrocyte. In order to evaluate the hemolysis of fibrous sepiolite from Guangji of Hubei Province, the fibrous sepiolite was treated by cation exchange, Gamble solution, organic acid and nicotine, and then the changes of the percentage of hemolysis and malandialdehyde (MDA)———the main product of superoxidation of lipids———before and after the treatment were observed. The results indicate the following: the RBC toxicity of fibrous sepiolite treated by cation exchange and Gamble solution decreases significantly and its possible mechanism is to decrease the intensity of lipid superoxidation and prolong the time of surface/membrane formation; the RBC toxicity of fibrous sepiolite increases significantly in the presence of nicotine; while fibrous sepiolite treated by organic acid has insignificant effect on human erythrocyte.