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PM2.5降尘对大鼠的急性肺损伤及复合维生素B的干预作用研究
Acute lung injury induced in rats by PM2.5 dustfall exposure and its intervention effects by vitamin B complex
投稿时间:2019-07-02  
中文关键词:复合维生素B  PM2.5降尘  急性肺损伤  干预
英文关键词:vitamin B complex  PM2.5 dustfall  acute lung injury  intervention effects
基金项目:国家自然科学基金重点项目(41831285);泸州市-西南医科大学联合项目(2017LZXNYD-J24);四川省教育厅科研项目(17ZA0431);西南医科大学基金资助计划(2017-ZRQN-002);国家级大学生创新创业训练计划项目(201710632054,201810632097)
作者单位E-mail
唐兰兰 西南医科大学公共卫生学院, 四川 泸州 646000  
邓胤铭 西南医科大学公共卫生学院, 四川 泸州 646000  
付茜茜 西南医科大学公共卫生学院, 四川 泸州 646000  
扈正权 泸州市环境监测中心站, 四川 泸州 646000  
张青碧 西南医科大学公共卫生学院, 四川 泸州 646000  
霍婷婷 西南科技大学固体废物处理与资源化教育部重点实验室, 四川 绵阳 621000  
柏珺 西南医科大学公共卫生学院, 四川 泸州 646000 baijunjill@gmail.com 
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中文摘要:
      为探讨复合维生素B(叶酸、B6和B12)能否抑制PM2.5对大鼠的急性肺损伤作用,本研究将56只SD大鼠随机分成对照组、不同剂量(0.4、2.0、10.0 mg/mL)PM2.5染毒组和相应的复合维生素B干预组(0.02 mg/mL叶酸、1 mg/mL维生素B6、0.002 5 mg/mL维生素B12),取肺组织进行HE染色观察组织病理学形态变化并进行病理学评分;收集支气管肺泡灌洗液(BALF)测定总蛋白(TP)、乳酸脱氢酶(LDH)、碱性磷酸酶(AKP)、酸性磷酸酶(ACP)、丙二醛(MDA)、超氧化物歧化酶(SOD)和过氧化氢酶(CAT)的含量。结果显示,PM2.5染毒能对大鼠肺组织造成不同程度的病理性损伤,复合维生素B能减缓此损伤;与对照组相比,低、中、高剂量的PM2.5染毒大鼠可引起BALF中LDH、AKP、ACP、TP和MDA含量显著升高(P<0.05),而SOD和CAT活性显著降低(P<0.05),且TP和MDA含量随染毒剂量的增加而升高(P<0.05),CAT活性随染毒剂量的增加而降低;3个染毒剂量的复合维生素B干预组LDH、TP和MDA含量显著降低(P<0.05),而SOD和CAT活性显著升高(P<0.05);PM2.5低、高剂量+复合维生素B组BALF中ACP含量显著降低(P<0.05);PM2.5中剂量+复合维生素B组BALF中ACP含量无显著改变;仅有PM2.5低剂量+复合维生素B组AKP含量降低(P<0.05),其余干预组AKP含量无显著变化。因此认为,PM2.5降尘能打破机体的氧化-抗氧化平衡,造成大鼠急性肺损伤,而复合维生素B能减缓此损伤,对机体起到保护作用。
英文摘要:
      To explore whether vitamin B complex (folic acid, B6 and B12) can inhibit the acute lung injury of PM2.5 in rats, the authors randomly divided 56 SD rats into control group, different doses (0.4, 2.0, 10.0 mg/mL) of PM2.5 exposure groups and corresponding vitamin B complex intervention groups (0.02 mg/mL folic acid, 1 mg/mL vitamin B6, 0.002 5 mg/mL vitamin B12). The lung tissue of rats was taken for HE staining to observe histopathological changes and calculate pathological scores. The bronchoalveolar lavage fluid (BALF) was collected to determine the content of total protein (TP), lactate dehydrogenase (LDH), alkaline phosphatase (AKP), acid phosphatase (ACP), malondialdehyde (MDA), superoxide dismutase (SOD) and Catalase (CAT). The results showed that PM2.5 exposure could cause different degrees of pathological damages of lung tissue in rats, and vitamin B complex could alleviate those damages. Compared with the control group, the content of LDH, AKP, ACP, TP and MDA in BALF of low, medium and high dose of PM2.5 groups significantly increased (P<0.05), while the activity of SOD and CAT significantly decreased (P<0.05). The content of TP and MDA increased with the increase of PM2.5 doses (P<0.05). The content of LDH, TP and MDA significantly decreased in the intervention groups of three PM2.5 doses (P<0.05), while SOD and CAT activity significantly increased (P<0.05). The content of ACP was significantly reduced in BALF in low and high dose of PM2.5+vitamin B complex groups (P<0.05). There was no significant change in ACP content in BALF in the medium dose of PM2.5+vitamin B complex group. Only the low dose of PM2.5+vitamin B complex group exhibited a decrease in AKP content (P<0.05), and the other intervention groups showed no significant change in AKP content. Therefore, it is held that PM2.5 could break the pulmonary oxidation-antioxidant balance to cause acute lung injury in rats, while vitamin B complex could alleviate the damage to protect the body.
唐兰兰,邓胤铭,付茜茜,扈正权,张青碧,霍婷婷,柏珺,2019,PM2.5降尘对大鼠的急性肺损伤及复合维生素B的干预作用研究[J].岩石矿物学杂志,38(6):852~860.
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