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温石棉和陶瓷纤维致大鼠炎症及氧化应激的毒性效应
Toxic effects of chrysotile asbestos and ceramic fibers on inflammation and oxidative stress in rats
投稿时间:2019-04-15  
中文关键词:温石棉  陶瓷纤维  炎症  氧化应激
英文关键词:chrysotile asbestos  ceramic fibers  inflammation  oxidative stress
基金项目:国家自然科学基金项目(41472046);四川省教育厅科研项目(17ZA0431);泸州市科技计划项目(2017LZXNYD-J24);西南医科大学项目(2017-ZRQN-002)
作者单位E-mail
黄柳雯 西南医科大学 药学院, 四川 泸州 646000  
崔琰 西南医科大学 公共卫生学院, 四川 泸州 646000  
查雨欣 四川省疾病预防控制中心, 四川 成都 610041  
柏珺 西南医科大学 公共卫生学院, 四川 泸州 646000  
董发勤 西南科技大学, 四川 绵阳 621010  
邓建军 绵阳四〇四医院, 四川 绵阳 621000  
张青碧 西南医科大学 公共卫生学院, 四川 泸州 646000  
王国俊 西南医科 大学附属医院药学部, 四川 泸州 646000 renren333@126.com 
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中文摘要:
      探讨了温石棉和陶瓷纤维致Wistar大鼠肺部炎症反应和氧化应激的毒性效应。将54只初断乳Wistar大鼠随机分为3组,即温石棉染毒组、陶瓷纤维染毒组和阴性对照组。采用非暴露式气管内滴注进行染毒(2.0 mg/mL,1次/月),并于1、6、12个月分批处死6只,观察肺组织病理学改变以及支气管肺泡灌洗液(BALF)和肺组织相关指标。结果显示,染毒组均出现不同程度的病理改变,其BALF中白细胞总数在染毒各时间点均高于阴性对照组(p<0.05);染毒组白细胞总数、中性粒细胞和淋巴细胞的百分比随染毒时间延长而升高(p<0.05),巨噬细胞百分比则下降(p<0.05);染毒组肺组织中IL-6、TNF-α、NF-κB含量在染毒各时间点均高于阴性对照组并随染毒时间延长而升高(p<0.05),且温石棉组高于陶瓷纤维组(p<0.05);染毒组肺组织中ROS、MDA浓度在染毒各时间点均高于阴性对照组并随染毒时间延长而升高(p<0.05),且温石棉组高于陶瓷纤维组(p<0.05);染毒组SOD活力均随染毒时间延长而下降(p<0.05),染毒一个月时与阴性对照组比较无差异(p>0.05),染毒6个月和12个月时温石棉组低于陶瓷纤维组且均低于阴性对照组(p<0.05)。综上,温石棉和陶瓷纤维均能对大鼠肺组织造成毒性作用,可引起炎症反应和氧化应激;温石棉致大鼠肺毒性的能力较陶瓷纤维强。
英文摘要:
      To explore the toxic effects of chrysotile asbestos and ceramic fibers on pulmonary inflammation and oxidative stress in Wistar rats, the authors randomly divided early weaning clean level of Wistar rats into 3 groups, namely, chrysotile asbestos exposure group, ceramic fibers exposure group and negative control group. The Wistar rats were administered by intratracheal instillation of chrysotile asbestos and ceramic fibers at the concentration of 2.0 mg/mL once a month. And 6 rats were sacrificed at 1, 6, and 12 months to observe pathological changes of lung tissues, bronchoalveolar lavage fluid (BALF) and lung tissues related indicators. The results showed that there were different degrees of pathological changes in the exposed groups, and the total number of white blood cells in BALF of exposed groups was higher than that in the negative control group at each time point (p<0.05). The percentage of white blood cells, neutrophils and lymphocytes in the exposed group increased with the prolonged exposure time (p<0.05), and the percentage of macrophages decreased (p<0.05). The content of interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α) and nueclear factor-κB (NF-κB) in lung tissues of the exposed groups was higher than that in the negative control group at each time point and increased with the prolonged exposure time (p<0.05), and the content in the chrysotile asbestos exposure group was higher than that of the ceramic fiber exposure group (p<0.05). The concentrations of reactive oxygen species (ROS), malondialdehyde (MDA) in lung tissues of the exposed groups were higher than those in the negative control group at each time point and increased with the prolonged exposure time (p<0.05), and those in the chrysotile asbestos exposure group were higher than the ceramic fiber exposure group (p<0.05). The superoxide dismutase (SOD) activity of the exposed groups decreased with the prolonged exposure time (p<0.05); there was no difference between exposed groups and the negative control group at 1 month (p>0.05); those in the chrysotile asbestos exposure group were lower than those in the ceramic fibers exposure group and lower than those in the negative control group at 6 and 12 months (p<0.05). All these results prove that chrysotile asbestos and ceramic fibers could cause toxic effects of inflammation and oxidative stress in lung tissues of rat, and chrysotile asbestos induced toxicity was stronger than ceramic fibers.
黄柳雯,崔琰,查雨欣,柏珺,董发勤,邓建军,张青碧,王国俊,2019,温石棉和陶瓷纤维致大鼠炎症及氧化应激的毒性效应[J].岩石矿物学杂志,38(6):834~842.
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