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PM2.5降尘对A549细胞线粒体氧化损伤的影响
Mitochondrial oxidative damage in A549 cells induced by PM2.5 dust-fall
投稿时间:2017-09-01  修订日期:2018-05-18
中文关键词:PM2.5降尘  A549细胞  ROS  MMP  氧化损伤
英文关键词:dustfall (PM2.5)  A549 cell  ROS  MMP  oxidative damage
基金项目:国家自然科学基金资助项目(41472046,41602033);四川省科技计划资助项目(2016JY0045)
作者单位E-mail
宋小芳 四川绵阳四○四医院, 四川 绵阳 621000  
杨洁 四川绵阳四○四医院, 四川 绵阳 621000  
霍婷婷 西南科技大学, 四川 绵阳 621010  
马骥 四川绵阳四○四医院, 四川 绵阳 621000  
董发勤 西南科技大学, 四川 绵阳 621010  
马杰 西南科技大学, 四川 绵阳 621010  
王玉琳 西南医科大学, 四川 泸州 646000  
邓建军 四川绵阳四○四医院, 四川 绵阳 621000 jianjundeng0801@163.com 
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中文摘要:
      将不同浓度PM2.5降尘作用于A549细胞后,利用MTT法检测其存活率,W-G染色观察细胞形态,荧光探针法检测细胞ROS和MMP相对水平,以探讨PM2.5降尘对A549细胞线粒体氧化损伤的影响及作用机制。结果显示,经12.5 μg/mL的PM2.5降尘作用A549细胞3 h后,细胞存活率为(81.77±6.15)%,并随作用浓度及时间增加呈递减趋势。PM2.5染毒后可观察到细胞形态不规则,胞膜溶解破坏,细胞微核出现。PM2.5降尘作用于细胞24 h后,胞内ROS相对含量随暴露浓度增加呈递增趋势,细胞MMP相对水平随染毒浓度增加而降低,且胞内ROS和MMP两者间存在显著相关关系(R2=0.878)。提示PM2.5降尘处理A549细胞后可通过刺激ROS的产生,诱导细胞MMP下降,造成细胞线粒体氧化损伤。
英文摘要:
      In this study, A549 cells were exposed to PM2.5 dustfall of different concentrations, respectively. MTT assay was used to evaluate cell viability. Cell morphology was observed through microscope after W-G staining. Fluorescence probe DCFH-DA and JC-1 were applied to label A549 cells after being treated by PM2.5 to detect intracellular level of ROS and change of MMP, with the purpose of investigating the mitochondrial oxidative damage in A549 cells induced by PM2.5 dust-fall. The results showed that, compared with the control group, A549 cell viability was (81.77±6.15)% after being exposed to PM2.5 at 12.5 μg/mL for 3 h, and was declined in a dose-dependent manner and time-dependent effect. It was observed that cell morphology was changed, cell membrane was dissolved and cell micro-nucleus appeared after being treated with PM2.5. In addition, the ROS levels were significantly higher than those of the control group; with the increase of PM2.5 concentration, the fluorescence intensity showed an increasing tendency gradually. Simultaneously, the MMP levels were declined in a dose-dependent manner after being treated with PM2.5. And there was a negative correlation between the ROS level and MMP (R2=0.878). It is inferred that PM2.5 may stimulate the production of ROS and induce the declining of MMP, resulting in A549 cell mitochondrial oxidative damage.
宋小芳,杨洁,霍婷婷,马骥,董发勤,马杰,王玉琳,邓建军,2018,PM2.5降尘对A549细胞线粒体氧化损伤的影响[J].岩石矿物学杂志,(6):985~992.
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