• 首页|本刊简介|在线投稿|过刊浏览|高级检索|专家风采|学习园地|期刊论坛|联系我们
我国四大矿区温石棉粉尘诱导A549细胞凋亡和周期阻滞的对比研究
A comparative study of A549 cell apoptosis and cell cycle arrest induced by chrysotile asbestos dust in four major mining areas of China
投稿时间:2017-08-31  修订日期:2018-04-25
中文关键词:温石棉  A549细胞  凋亡  周期阻滞
英文关键词:chrysotile asbestos  A549 cell  apoptosis  cell cycle arres
基金项目:国家自然科学基金项目(41472046,41602033);四川绵阳市科技计划项目(13S002-5)
作者单位E-mail
黄凤德 四川绵阳四○四医院 四川 绵阳 621000  
王玉琳 西南医科大学临床医学院 四川 泸州 646000  
冯晨旭 西南科技大学 环境与资源学院, 四川 绵阳 621010  
霍婷婷 西南科技大学 固体废物处理与资源化教育部重点实验室, 四川 绵阳 621010
西南科技大学 环境与资源学院, 四川 绵阳 621010 
 
董发勤 西南科技大学 固体废物处理与资源化教育部重点实验室, 四川 绵阳 621010
西南科技大学 环境与资源学院, 四川 绵阳 621010 
 
刘琬薰 西南医科大学临床医学院 四川 泸州 646000  
杨洁 西南医科大学临床医学院 四川 泸州 646000  
曾娅莉 四川绵阳四○四医院 四川 绵阳 621000  
邓建军 四川绵阳四○四医院 四川 绵阳 621000
西南医科大学临床医学院 四川 泸州 646000 
jianjundeng0801@163.com 
摘要点击次数: 29
全文下载次数: 32
中文摘要:
      通过FT-IR和XRD对四川新康、陕西陕南、甘肃阿克塞和青海茫崖四大矿区温石棉的物相成分、谱学特征进行测试分析,利用MTT法、流式细胞术检测了4种温石棉纤维粉尘染毒A549细胞后的细胞存活率、凋亡率和细胞周期分布,比较和探讨了四大矿区温石棉纤维粉尘抑制细胞增殖能力的大小及其对细胞凋亡和周期阻滞的影响。研究结果显示,四大矿区温石棉的主要物相是斜纤蛇纹石,新康温石棉成分比其他3种温石棉的复杂,阿克塞和茫崖温石棉的成分相似;四大矿区的温石棉化学基团基本相同,阿克塞和茫崖温石棉纤维的表面结构较陕南和新康温石棉的完善。4种温石棉均能不同程度地抑制A549细胞增殖,诱导细胞凋亡和周期阻滞:茫崖组凋亡率最大(22.56±2.56)%,陕南组凋亡率最小(19.57±3.07)%;细胞周期阻滞均以G2/M期阻滞为主,新康、陕南、阿克塞和茫崖组处于G2/M期细胞比例分别增加8.90%、8.58%、9.13%和10.44%。4种温石棉导致细胞凋亡和周期阻滞的能力:茫崖>新康>阿克塞>陕南。
英文摘要:
      The compositions of chrysotile asbestos in four major mining areas of Xinkang in Sichuan, Shannan in Shaanxi, Akesaic in Gansu and Mangya in Qinghai were analyzed by FTIR, their cell viability was detected by MTT assay, and their cell apoptosis rate and cell cycle arrest were tested by flow cytometry. The authors compared and discussed the effects of chrysotile asbestos dust on cell proliferation, cell apoptosis and cell cycle arrest in four mining areas. The results show that the main phase of chrysotile asbestos in four mining areas was oblique fiber serpentine, the composition of Xinkang chrysotile asbestos was more complex than that of the other three kinds of chryso-tile asbestos, and the composition of Akesai is similar to that of the Mangya chrysotile asbestos. The chemical groups of chrysotile asbestos in the four major mining areas are basically the same, and the surface structure of Akesai and Mangya chrysotile fibers is better than that of Shannan and Xinkang. Four kinds of chrysotile asbestos can inhibit the proliferation of A549 cells in different degrees, inducing apoptosis and cell cycle arrest:the maximum apoptosis rate is (22.56±2.56)% in Mangya group, the lowest apoptosis rate is (19.57±3.07)% in the Shannan group; and the cell cycle arrest is mainly at G2/M stage. The proportions of G2/M stage (Xinkang, Shannan, Akesai, Mangya) are increased by 8.90%, 8.58%, 9.13% and 10.44% respectively. The capability of four kinds of chrysotile asbestos for inducing cell apoptosis and cell cycle arrest is in order of Mangya > Xinkang > Akesa > Shannan.
黄凤德,王玉琳,冯晨旭,霍婷婷,董发勤,刘琬薰,杨洁,曾娅莉,邓建军,2018,我国四大矿区温石棉粉尘诱导A549细胞凋亡和周期阻滞的对比研究[J].岩石矿物学杂志,(6):967~974.
查看全文  查看/发表评论  下载PDF阅读器
关闭
您是本站第 4993919位访问者  京ICP备05032737号-8
版权所有:《岩石矿物学杂志》编辑部
主管:中国科学技术协会 主办:中国地质学会岩石学专业委员会 中国地质学会矿物学专业委员会 中国地质科学院地质研究所
技术支持:北京勤云科技发展有限公司

京公网安备 11010202007772号

function PdfOpen(url) { var win="toolbar=no,location=no,directories=no,status=yes,menubar=yes,scrollbars=yes,resizable=yes"; window.open(url,"",win); } function openWin(url,w,h){ var win="toolbar=no,location=no,directories=no,status=no,menubar=no,scrollbars=yes,resizable=no,width=" + w + ",height=" + h; controlWindow=window.open(url,"",win); } &et=8E4FC607531F30FE26E3E977424F764A33BEACBB95F18F0FD2B3CA8F8F2459A83062ADB2CE01000A4C3A20A5F516A7D03BAF08CC21ECDD9389121BC4C86924C2B4F70C241BF247A53F123881194CCE41E41C2E4638A6C88C1BA35D0A8FB9C4F14670C7031AF6E38F4D3E92B040FEE63B1AA188FAD6D2A07201027F27A93BBF79F892F57E4A05406441D8A032A8263E96&pcid=E62459D214FD64A3C8082E4ED1ABABED5711027BBBDDD35B&cid=621CF755B1A341E5&jid=25F5F0F8F42BD24D151314EDEA002993&yid=EA357AD73C8E13BC&aid=&vid=&iid=B31275AF3241DB2D&sid=F131C0ADC94A25CF&eid=3A9B6DFDAB34D091&fileno=20180608&flag=1&is_more=0"> var my_pcid="E62459D214FD64A3C8082E4ED1ABABED5711027BBBDDD35B"; var my_cid="621CF755B1A341E5"; var my_jid="25F5F0F8F42BD24D151314EDEA002993"; var my_yid="EA357AD73C8E13BC"; var my_aid="";