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基于多次非暴露式气管滴注的染毒法研究温石棉致大鼠肺损伤的作用
The lung injury induced by chrysotile asbestos in rats based on the multiple non-exposed intratracheal instillation
投稿时间:2017-08-31  修订日期:2017-10-10
中文关键词:多次非暴露式气管滴注  温石棉  Wistar大鼠  肺损伤
英文关键词:multiple non-exposed intratracheal instillation  chrysotile asbestos  Wistar rats  lung injury
基金项目:国家自然科学基金面上项目(41472046);四川省科技厅自然科学基金(14JC0126);西南医科大学基金资助计划(2015-YJ124)
作者单位E-mail
柏珺 西南医科大学 公共卫生学院, 四川 泸州 646000  
崔琰 西南医科大学 公共卫生学院, 四川 泸州 646000  
张青碧 西南医科大学 公共卫生学院, 四川 泸州 646000  
霍婷婷 西南科技大学, 四川 绵阳 621000  
查雨欣 西南医科大学 公共卫生学院, 四川 泸州 646000  
邓建军 四川绵阳四○四医院 检验科, 四川 绵阳 621000 jianjundeng0801@163.com 
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中文摘要:
      温石棉是一种安全性存在争议的石棉,合理可行并且能够真实反映其暴露致病过程的染毒方法对深入研究其致病机理有着重要意义。本文采用多次非暴露式气管滴注的染毒方法对雄性Wistar大鼠分别进行不同浓度温石棉悬液的染毒,在染毒1、3和6个月后记录体重和肺脏器系数,观察肺组织大体及苏木素-伊红(HE)染色的病理形态,对支气管肺泡灌洗液(BALF)中白细胞总数进行分类计数,并测定总蛋白(TP)、乳酸脱氢酶(LDH)、酸性磷酸酶(ACP)和碱性磷酸酶(AKP)的含量。结果表明,多次滴注的方式大鼠死亡率较低,并且每次滴注后能够苏醒的大鼠没有死亡发生;随着四川新康温石棉染毒时间的延长和染毒剂量增加,大鼠体重增长缓慢,肺脏器系数升高,肺大体出现水肿、肉芽肿、萎缩实质化,HE染色观察则表现为炎性细胞增多、肺泡结构遭到破坏以及出现纤维化;随着染毒时间的延长,支气管肺泡灌洗液(BALF)中的白细胞总数、中性粒细胞和淋巴细胞百分比呈上升趋势,巨噬细胞百分比呈下降趋势,TP、LDH、ACP和AKP均表现为上升的趋势,并呈现一定的剂量-效应关系。综上所述,多次非暴露式气管滴注方法可用于模拟温石棉慢性暴露的致病过程,四川新康温石棉可以通过破坏巨噬细胞、肺泡上皮细胞及肺泡上皮-毛细血管屏障造成大鼠的肺损伤。
英文摘要:
      Chrysotile asbestos is a kind of asbestos that is controversial in safety, and it is of great significance to study its pathogenesis in a reasonable and feasible way to reflect its exposure process. A multiple non-exposed intratracheal instillation method was used to expose male Wistar rats under the condition of different concentrations of chrysotile asbestos. After exposure for 1, 3 and 6 months, the rats' body weight and lung coefficient were recorded, and pulmonary tissue and HE Staining slices were observed. The brochoalveolar lavage fluid (BALF) was collected to measure the change of different kinds of white cells, total protein (TP), lactate dehydrogenase (LDH), acid phosphatase (ACP) and alkaline phosphatase (AKP). The results showed that a low mortality was presented by using the multiple intratracheal instillation method. The body weight increased slowly and lung coefficient increased with the time and dosage. The edema, granuloma and atrophy appeared in pulmonary tissue. The increased inflammatory cells, destroyed alveolar and fibrosis were observed by HE staining. Chrysotile asbestos induced the increase of total white cells, the neutrophils and lymphocytes percentages, but the reduction of the macrophages percentage in BALF. The values of TP, LDH, ACP and AKP in exposure groups were higher than those in control group with a significant dose-response correlation. All these results prove that the multiple intratracheal instillation method could be used to simulate the chronic exposure of chrysotile asbestos. The Xinkang chrysotile asbestos could induce lung injury through destroying the lymphocytes, alveolar epithelial cells and alveolar epithelial-capillary barrier.
柏珺,崔琰,张青碧,霍婷婷,查雨欣,邓建军,2017,基于多次非暴露式气管滴注的染毒法研究温石棉致大鼠肺损伤的作用[J].岩石矿物学杂志,36(6):916~924.
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